3D Biosilica Scaffolds from Melophlus sarasinorum and Xestospongia testudinaria Indonesian Sponges are Biocompatible for Cell Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cell in Bone Tissue Engineering

BACKGROUND: Biosilica derived from Indonesian marine sponge Melophlus sarasinorum and Xestospongia testudinaria is one of the biomaterials that can be developed together with synthetic polymer as a composite. Poly E-caprolactone (PCL) used composite role an osteoconductive material biosilica also tailored slow rate degradation in body. This study aimed to create biocompatible biosilica-based scaffold supports osteogenic differentiation human Wharton's Jelly mesenchymal stem cell (hWJ-MSCs).METHODS: was extracted M. X. acid digestion method. Scaffold prepared using salt leaching The scaffolds were made seven different extract PCL. All tested for morphology, Fourier-transform infrared spectroscopy (FTIR), immunocytochemistry, cytotoxicity.RESULTS: Composite 50% increased viability supported growth within 14 days, whereas seen by presence collagen type 1 day 12 based on immunocytochemistry result.CONCLUSION: PCL+50% promising 3D potential application bone tissue engineering. In conclusion, this shows evidence hWJ-MSC, which might engineering.KEYWORDS: sponge, biosilica, scaffold, osteogenesis,